[Xenopus] pCS-GAP43-GFP sequence

[Evans, Sylvia]

Hi Frank

think you might have sent this to me by mistake?

hope all goes well with you.

all the best

sylvia


On Jun 11, 2014, at 11:12 AM, "Conlon, Frank L" <frank_conlon at med.unc.edu<mailto:frank_conlon at med.unc.edu>> wrote:

Fully agree with Sally.

On Jun 11, 2014, at 2:08 PM, "Sally Moody" <samoody at gwu.edu<mailto:samoody at gwu.edu>> wrote:

Hi Jill,
We don't have that sequence, but in our experience it is better to send it out for sequencing rather than rely on somebody's map because things are not always what they seem. Since it is in pCS, you can use M13 primers or better yet SP6 and T7 primers for that plasmid.
Good luck, Sally


On Wed, Jun 11, 2014 at 1:46 PM, Jill Johnston <johnstoj at ucalgary.ca<mailto:johnstoj at ucalgary.ca>> wrote:
Hello,

We have a construct called pCS-GAP43-GFP here and have no information
about it.  Does anyone have the sequence that could be sent to us?

Thanks


Jillian Johnston
Research Associate
Dr. Sarah McFarlane lab
University of Calgary
Faculty of Medicine
Dept of Cell Biology and Anatomy




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Sally A. Moody, Ph.D.
Professor of Anatomy and Regenerative Biology
George Washington University
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