[Xenopus] MISC

[Green, Jeremy]

Dear Leon,

With a bit of practice and well-sharpened Dumont #5 forceps, a person can cut around 200 caps in an hour. I suggest that if you want to get many hundreds of caps that you simply have a cap-cutting jamboree - not so much biting the bullet as having a party. Seriously. I think you’ll have no problem getting volunteers and (COVID, etc. permitting), I’d be happy to come over myself.

Best wishes,

Jeremy

Jeremy B.A. Green Ph.D.
Vice-Dean International (Research)
Professor of Developmental Biology
Centre for Craniofacial & Regenerative Biology
King's College London
Guy's Tower, Floor 27
London SE1 9RT
UK

jeremy.green at kcl.ac.uk<mailto:jeremy.green at kcl.ac.uk>
Tel. +44 20 7188 1794
https://www.kcl.ac.uk/people/jeremy-green





On 29 Oct 2020, at 03:45, Leon Peshkin <peshkin at gmail.com<mailto:peshkin at gmail.com>> wrote:

Dear Colleagues !

I have a few general questions and tried posting to Slack "Xenopus Community" without success. People - Slack is a nice way to keep discussions going, please use it !

Has someone worked out a way to make animal caps en masse ?  I did hear the gastromaster legend and even obtained one with a few tips … but it does not really work in my hands. Any other devices ?  Please do not tell me to just bite the bullet. I'd like to be able to cut many hundreds.
   There are a couple of animal cap instructional videos with forceps but I know many people use other techniques. Would be nice to have more videos.

Does someone have a protocol and experience for blood perfusion in Xenopus ?

   THANK YOU

   - L. Peshkin


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