[Xenopus] MISC

[Peter Vize]

Hi Leon

Yes, they have. John Wallingford and Ed Marcotte scaled it up a few years ago to do protein:protein work. I’m sure John can provide the details. They use gastromasters and worked out how to make their own tips.

Slack takes over your life and I avoid it like the plague…

Peter


Dr. Peter Vize
Professor Emeritus
Department of Biological Sciences
University of Calgary
2500 University Drive NW
Calgary, Alberta T2N 1N4
Canada

ph +61 401 300 965

http://www.vizelab.com/

On Oct 29, 2020, at 2:45 PM, Leon Peshkin <peshkin at gmail.com<mailto:peshkin at gmail.com>> wrote:

[△EXTERNAL]


Dear Colleagues !

I have a few general questions and tried posting to Slack "Xenopus Community" without success. People - Slack is a nice way to keep discussions going, please use it !

Has someone worked out a way to make animal caps en masse ?  I did hear the gastromaster legend and even obtained one with a few tips … but it does not really work in my hands. Any other devices ?  Please do not tell me to just bite the bullet. I'd like to be able to cut many hundreds.
   There are a couple of animal cap instructional videos with forceps but I know many people use other techniques. Would be nice to have more videos.

Does someone have a protocol and experience for blood perfusion in Xenopus ?

   THANK YOU

   - L. Peshkin


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