[Xenopus] MISC

[John Wallingford]

Hi all,

It’s nice to know that my name is now invoked in mystical legends…..

As it happens, we TRIED to make the gastromaster work for en mass cutting of caps, but we were not successful.  Ultimately, the tips get gummed.

Leon, I know you don’t wanna hear it, but…just bite the bullet.  My folks now routinely cut >1000 caps or DMZs in a single session, but they do it the old fashioned way:  Three people, six pairs of Inox #5, three stereoscopes, and several hours…..

And sorry, I don’t do Slack.

-jbw




——————————————————————
John Wallingford
William and Gwyn Shive Endowed Professor
Dept. of Molecular Biosciences
University of Texas at Austin
512-232-2784
wallingford at austin.utexas.edu<mailto:wallingford at austin.utexas.edu>







On Oct 29, 2020, at 2:50 PM, Peter Vize <pvize at ucalgary.ca<mailto:pvize at ucalgary.ca>> wrote:

Hi Leon

Yes, they have. John Wallingford and Ed Marcotte scaled it up a few years ago to do protein:protein work. I’m sure John can provide the details. They use gastromasters and worked out how to make their own tips.

Slack takes over your life and I avoid it like the plague…

Peter


Dr. Peter Vize
Professor Emeritus
Department of Biological Sciences
University of Calgary
2500 University Drive NW
Calgary, Alberta T2N 1N4
Canada

ph +61 401 300 965

http://www.vizelab.com/

On Oct 29, 2020, at 2:45 PM, Leon Peshkin <peshkin at gmail.com<mailto:peshkin at gmail.com>> wrote:

[△EXTERNAL]


Dear Colleagues !

I have a few general questions and tried posting to Slack "Xenopus Community" without success. People - Slack is a nice way to keep discussions going, please use it !

Has someone worked out a way to make animal caps en masse ?  I did hear the gastromaster legend and even obtained one with a few tips … but it does not really work in my hands. Any other devices ?  Please do not tell me to just bite the bullet. I'd like to be able to cut many hundreds.
   There are a couple of animal cap instructional videos with forceps but I know many people use other techniques. Would be nice to have more videos.

Does someone have a protocol and experience for blood perfusion in Xenopus ?

   THANK YOU

   - L. Peshkin


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