[Xenopus] New centriole transgenic lines

National Xenopus Resource xenopus at mbl.edu
Wed May 30 16:19:08 EDT 2018

Dear Xenopus community
            I am pleased to announce we have two new centriole transgenic lines that were generated for Brian Mitchell and which he has kindly made available to the community prior to publication. These two lines are:
             Tubulin promoter driving expression of Deup1 fused to eGFP labeling both deuterosomes and centrioles specifically in multi-ciliated cells.  This is a great marker for visualizing centrioles.

                CMV promoter driving expression of centrin 4 (cetn4) fused with RFP labeling centrioles in all cells.  Note: while the expression level and centriole fluorescent intensity is quite high in most cells, the number of centrioles in multi-ciliated cells appears to dilute the intensity/centriole.

Marko Horb

If the NXR contributed to your work please cite us in your papers.
Cite us in Materials and Methods as National Xenopus Resource RRID:SCR_013731 at the Marine Biological Laboratory
Cite us in your references: Pearl, E. et al "Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center" Genesis 50, 155-163

Marko Horb, Ph.D. |Director, National Xenopus Resource (NXR) |Senior Scientist, Bell Center for Regenerative Biology and Tissue Engineering
Marine Biological Laboratory | 7 MBL Street | Woods Hole, MA 02543
Email: mhorb at mbl.edu<mailto:mhorb at mbl.edu> | Office: 508-289-7627 | Lab: 508-289-7370 | Cell:  508-564-3764


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